Objective In order to provide scientific basis for Brucellosis control and prevention, sero-epidemiological survey, Brucella isolation and genetic characteristic analysis were conducted for the high-risk population in a human Brucellosis epidemic in Weining county of Guizhou province. Methods Tube agglutination test was used to detect the anti-Brucella antibody for the high-risk human population. Rose Bengal Plate Test was applied to detect the antibody in goat blood samples. The blood of antibody positive human population was collected for Brucella isolation. Conventional methods, genus specific BCSP31-PCR and species-specific AMOS-PCR were used to identify the bacteria strain, and the genetic characteristic was analyzed using MLVA and MLST techniques. Results Six people of 43 high-risk populations were confirmed as anti-Brucella antibody positive, 64 out of 302 goat blood samples were anti-Brucella antibody positive, with positive rate of 21.19%. A suspected Brucella strain were isolated from one of the high-risk human populations and was identified as B. melitensis biovar 3. MLVA-16 analysis indicated that the bacteria strain was most closely clustered with B. melitensis biovar 3. Multilocus sequence typing analysis indicated the strain was ST8. Conclusion Genetic characteristic of Brucella strain isolated from the Brucelosis epidemic was consistent with that of M. melitensis biovar 3. Although antibody and Brucella were detected, the high-risk populations did not displayed symptoms, so all of them were asymptomatic infections. The epidemic was seemingly imported due to goats trading, suggested that health and animal disease control and prevention departments and doctors should pay great attention to it.

Objective Borrelia burgdorferi were isolated from captured ticks in the border areas between Guangxi and Guizhou, in order to identify the vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province. Methods The BSK?Ⅱ culture media were used to isolate B. burgdorferi from ticks. The 5S-23S rRNA gene spacer region of the isolates was amplified by the PCR, and the product was cloned and sequenced. The sequencing results were compared with the 5S-23S rRNA gene spacer region of the B. burgdorferi strains from the gene bank and analyzed for the homology to identify their genotypes. Results One B. burgdorferi was isolated from the captured Ixodes sinensis (named QLT1). The isolate was identified as the genotype B. valaisiana. Conclusion I. sinensis is the likely vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.

【Abstract】 Objective To investigate the influence factors in areas where rabies raged in Guizhou province, and provide the evidence for the establishment of focused intervention measure suitable for the rural areas. Methods A multistage sampling method was used in this study and all the data were analyzed by Excel 2003 after logging data with Epi Data. Results In recent years, the incidence of rabies in Zhenning county was very serious. The incidence rates of rabies in 2005 and 2006 were 6.16/10 thousand and 5.19/10 thousand respectively, which mainly caused by bite of dogs. Because of the large number of the dogs, the injury rate of the crowd was also higher. It was 6.47% in 2006, and there was significant difference between exposure rate of students （10.58%） and that of farmers （5.05%）（χ2＝36.57, P＜0.001）. About 31.40% exposed crowd were not done the medical treatment, and 35.41% exposed crowd without inoculation rabies vaccines, only 4.26% of Ⅲ° exposed crowd with rabies immunoglobulin. Villagers knew less about the knowledge of rabies prevention and control. Conclusion The main factors leading to the rage of rabies were large number of dogs, low immunization rate of the crowd, high exposure rate of the public and low treatment rate of exposed crowd in the investigated districts.

Objective To analyse prevalence trend of Japanese encephalitis(JE) in 1997-2004 in Guizhou province and to supply scientific gist for the prevention and Control strategy. Methods Analysis information was got from the epidemic surveillance and JE Cases Questionnaire. Results There were accumulative total 13 664 JE cases and 540 death cases. The annual average Morbidity and Mortality and fatality rate were 4.65/100 000 and 0.18/100 000 and 3.95%,respectively. Male to female was 1.48∶1. Children of decentralization resident population accounted for 67.54%( 9 228/ 13 664) and students were 23.06%( 3 151/ 13 664). Younger than 10 years old children case accounted for 88.00%( 12 024/ 13 664);Season distribution was from June to October and high peak period was from July to August. Conclusion The results showed that JE cases were mainly younger than 10 years old children in Guizhou province. The cases season were from June to October and high peak period lasted two months from July to August. The high risk prefectures were Zunyi and Bijie. There were so many cases with out vaccine history and unknown history during 1997-2004. The preventive strategies are to be that the health education and propaganda should be conducted. All-around control countermeasure such as preventive and destroying mosquitoes and JE vaccine immunization should be carried on to decrease the morbidity and mortality of JE in Guizhou province.

Objective Analyses epidemiological status of rabies and searches for the control countermeasure according to the rabies cases surveillance in Guizhou province in 2004.Methods Analyse synthetically information of epidemic situation surveillance administration system and rabies cases questionnaire form.Results Totally 206 rabies cases were reported in Guizhou province in 2004 and the morbidity was(0.53/100 000).Mortality was the same as morbidity.Among them,the rabies cases of Anlong county is account for the 65.67%((44/67)) of Qianxinan prefecture.Though there were rabies cases in the whole year,the peak was March and from July to October and the rabies cases from July to October amount for 46.12%((95/206).Proportion of male and female was 2.68∶1.The most cases were farmers and students which amount to 57.28% and 27.18% respectively.The high risk age was(10-44) years and amount to 66.5%.The youngest rabies case was 2 years old and the oldest was 76 years old.Conclusion Rabies cases mostly were farmers and students in Guizhou.The high peak season of rabies cases is summer and autumn.High risk prefecture is Qianxinan,Qiannan,Qiandongnan and Anshun city.Suggestion more works should be done according to "Administrating,Immunizing and Destroying" principle.Strengthen propaganda and health education,and let people realize the importance of injecting the rabies vaccine and immunize on one's own initiative.It will decline the rabies morbidity and mortality in Guizhou province and protect the health of people.